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The main objective is to provide breeders and molecular engineers with an understanding of the bottlenecks for successful reproductive development at high temperatures.
However, during the process of optimizing and realizing their full potential, it is pertinent to gain a basic mechanistic understanding of the bottlenecks existing for nanoparticle-mediated intracellular delivery.
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As this would severely limit the potential of L. lactis as an alternative expression platform for structural biology, we aimed at understanding and overcoming the bottlenecks limiting the functional expression of complex proteins upon growth in CDM.
However, a detailed understanding of the underlying transfer rates and potential bottlenecks is crucial for designing improved systems.
To distinguish selective traits from those caused by bottleneck effects, understanding of the population history that the first population captured from wild became domestic population has also been a vital task (Axelsson et al., 2013).
One of the bottlenecks in discovering appropriate cancer markers is a poor understanding of the pathophysiology of the disease [26], [27], [46].
However, the poor understanding of the switching reliability, mechanism, low-current operation (<100 μA) are the bottlenecks in its further development and optimization.
Increased understanding of the host organism biology has suggested engineering strategies targeting bottlenecks in transcription, translation, protein processing and secretory pathways, as well as cell growth and survival.
Besides modeling of the buildings, the bottleneck of MPC wide-spreading is the understanding of the MPC paradigm from the HVAC engineers and managers.
Thirdly, how to gain a deep understanding of the ocean ecosystem from these samples is still a bottleneck.
A thorough understanding of the factors that determine expression levels and strategies to overcome bottlenecks are important for predictable protein production.
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