Sentence examples for underside of the membrane from inspiring English sources

The number of cells that migrated out onto the underside of the membrane (invasion), or attached onto the collagen I-coated plastic surface (adhesion/viability), was scored by staining the cells with 1% crystal violet or Hoechst 33342, respectively.

Transwell membranes were removed and the underside of the membrane was imaged and nuclei counted using NIS-Elements (Nikon, Melville, NY) software.

After GFP-labeled RF/6A cells were placed in contact with an RPE monolayer plated on the underside of the membrane for 4 hrs, the number of invaded RF/6A cells was quantified and the results showed that the number of cells that over-expressed Dll4 was significantly lower than the number of pDNA-transfected cells.

The immunostaining demonstrates the underside of the membrane exhibited a purely axonal component.

The cells on the underside of the membrane were fixed and stained.

Upper wells were placed into 4% paraformaldehyde for 15 min to fix cells adherent to the underside of the membrane.

Migration was examined on 16-transwell plates (Roche Diagnostics GmbH) with microelectrodes attached to the underside bottom of the membrane for impedance-based detection of the migrated cells.

Cells on the underside of the membranes were fixed, stained with crystal violet, and counted in five independent microscopic fields.

Cells that migrated to the underside of the membranes were fixed and stained with Diff-Quik (Dade Behring) and counted.

Cells on the underside of the membranes were photographed (five fields per membrane at ×100 magnification) using an inverted microscope and camera (Axiovert 200, Carl Zeiss, Germany).

After incubation for 26 h, the cells, which migrated or invaded through the membrane alone or the Matrigel-coated membrane, respectively, and remained bound to the underside of the membranes, were stained with the Diff-Quik and counted in five random microscopic fields/filters.