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After 18 h, cells migrated to the underside of the insert were stained with crystal violet.
(C) Cells were transfected as in (A), and transwell migration experiments were performed and cells migrated to the underside of the insert were stained with crystal violet.
After 18 h, cells on the inside of the transwell insert were removed with a cotton swab, and cells on the underside of the insert were fixed with 4% paraformaldehyde and stained with crystal violet solution.
The invaded cells on the underside of the insert were fixed with methanol, stained with crystal violet for 2 min, and rinsed with phosphate buffered saline (PBS).
The total number of invading cells was determined by counting the cells on the underside of the insert from triplicate wells (six fields per insert) at 20× magnification.
After 72 (MCF7) and 48 (BT549 and BT474) hours of incubation, tumor cells that were able to degrade Matrigel™ and migrate through 8 um pores onto the underside of the insert were counted.
Similar(51)
Cells that had invaded to the underside of the inserts were counted by light microscopy.
Cells that had invaded through the underside of the inserts were counted by light microscopy.
Cells that had moved to the underside of the inserts were fixed with formaldehyde and stained with crystal violet.
Cells that had migrated to the underside of the inserts were stained with crystal violet and the cells on each insert were counted at 200× magnification.
The cells from the underside of the inserts were completely dislodged, lysed, and stained by Cyquant GR Dye (Molecular Probes, Inc).
More suggestions(15)
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underside of the neck
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