Sentence examples for underside of the filter from inspiring English sources

Invading cells on the underside of the filter were scored from eight random fields.

Cells in the bottom wells were then harvested by pipetting, and residual cells were collected by rinsing the underside of the filter with 10 mM EDTA in PBS.

After 24 h, invaded cells on the underside of the filter were methanol fixed and stained with 1% toluidine blue/1% borax solution.

The cells that migrated to the underside of the filter were enumerated from 5 to 10 random fields using DAPI fluorescence.

After a 24 hour incubation in a Matrigel-coated Transwell chamber, FLJ10540 CL1-0-stable transfectants had invaded the basement membrane material and passed through the pores to reach the underside of the filter (Figure 2B, left bottom panel).

Accordingly, a significant proportion of cells were not able to extend protrusions to the underside of the filter, and some APC clusters at the ends of microtubules are still present on the upper surface of the membrane (Fig. 4B).

Cells in the upper compartment were removed, leaving only the cells on the underside of the filter- these represented those cells who had successfully invaded across the collagen-coated filter.

Cells on the underside of the filters were examined and counted under a microscope.

Epifluorescence microscopy was performed to detect nuclei of cells that migrated to the underside of the filters.

Excess stain was washed away with water, the attached cells on the upper side of the filters were mechanically removed using wet, cotton-tipped applicators, and the migratory cells on the underside of the filters were quantitated by counting four high-powered fields (× 100 magnification) per well using an inverted light microscope (Olympus BH 2).

The undersides of the filters were fixed 6 hrs after the RF/6A cells were plated, and the migrated cells were counted under a fluorescence microscope.