Sentence examples for underside of the chamber from inspiring English sources

Following 4 h of incubation, cells were allowed to migrate to the underside of the chamber for another 4 h in the presence or absence of vascular endothelial growth factor (VEGF) (20 ng/ml) in the lower chamber.

The migrated or invaded cells were dislodged from the underside of the chamber using a detachment solution.

Cells suspended in serum-free media were added to each chamber and allowed to invade towards the underside of the chamber for 20 h at 37 °C.

Cells were added to the top of each migration chamber and allowed to migrate to the underside of the chamber for 24 h in the presence of 10% FCS in the lower chamber.

In each cycle, invading cells on the underside of the chamber were fixed with 10% formalin-neutralized buffer, stained with 0.1% crystal violet, and imaged through a microscope (Axiovert 200M; Carl Zeiss, Jena, Germany) with a CCD camera (AxioCam; Carl Zeiss).

Cell migration was assessed using double-chamber 16-well plates with microelectrodes located on the underside of the upper chamber's PET membrane containing 8.1 m pores (CIM-plate 16, Roche Applied Science).

Surprisingly, the NI populations displayed invasion similar to the isolated I populations, to the underside of the invasion chamber.

Figure 4(a) represents the levels of cell invasion to the underside of the invasion chamber (basic invasion).

Figure 5(a) represents the levels of cell migration to the underside of the motility chamber (basic motility).

Cells on the underside of the transwell chambers were collected by incubating the bottom of the chamber in 225 µl of Cell Dissociation Buffer (equal parts 0.5% phenol red-free Trypsin EDTA (Invitrogen) and Hank's Balanced Salt Solution (5.37 mM KCl, 0.45 mM KH2PO4, 137 mM NaCl, 4.17 mM NaHCO3, 0.34 mM Na2HPO4, 5.55 mM D-glucose)) for 20 minutes at 37°C, 5% CO2.

These plates are similar to conventional transwells with the microelectrodes located on the underside of the membrane of the upper chamber.