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Twenty-four and forty-eight hs later the cells of the upper surface of the filter were removed with a cotton swab; those underneath were fixed with 4% paraformaldehyde, stained with 0,2% crystal violet and counted.
Cells from the upper surface of the filter were removed with a cotton swab; those underneath were fixed with 4% paraformaldehyde prior to staining with 0.5% crystal violet.
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The sensors were fixed underneath the center of the flume bed through three 10 mm-diameter holes drilled on a horizontal line at the center of the flume bed.
A wedge of cartilage is positioned underneath and is fixed to the framework by sutures.
Keep doors shut when the heating is on, especially if you congregate in one room during the evenings, and dig out Granny's sausage dog – it's one of the best draught excluders going, although door draught excluders, which are fixed underneath, are also good.
A piezoelectric actuator (5) is fixed underneath the body of the cantilever array.
To improve stability and durability, a piece of cardboard may be fixed underneath the box using tape.
A plumb line was fixed underneath the Velcro strap attached around the subject's head and a small weight suspended from it (105 grams altogether), which hung to just short of the floor.
To correct the inversion of the foot, all of the foot distal to the talus must be made to rotate laterally underneath the talus which is fixed in the ankle mortise.
c A pectoralis major muscle flap was fixed to the fistula site, underneath the trachea.
That had to be fixed by adding fire rated boards underneath".
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