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A 2 mm high cylindrical polyethylene tube with an internal diameter of approximately 8 mm was placed on the surface of each underlying specimen, the tube was filled with composite (either FZ250 or FS), and all samples (except the self-cure samples) were polymerized for 20 s using an LED curing unit, with all procedures carried out at room temperature.
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Records of patients who had colonized or infected with Staphylococcus strains with decreased susceptibility to glycopeptides were reviewed and data related to the patients age, gender, ward, underlying disease, specimen, duration of hospitalization and previous treatment with glycopeptides were evaluated.
Underlying material specimens were prepared using plastic molds drilled with holes 10 ± 0.1 mm in diameter and 1 ± 0.1 mm in depth.
This technique involves circulating polyethylene glycol solution (PEG) over a semi-permeable membrane underlying a test specimen.
For each underlying material, 20 specimens were prepared, for a total of 80 specimens.
It has been found that although the crushing patterns that develop during the load plateau vary from specimen to specimen (influenced by geometric imperfections and by specimen size) the underlying cell collapse mechanism is common to all specimens.
Patients were treated in various departments and a large spectrum of age, clinical specimen, and underlying disease was observed.
OA cartilage (femoral condyles and tibial plateaus) was obtained under aseptic conditions and carefully dissected from the underlying bone in each specimen.
OA cartilage (femoral condyles and tibial plateaus) was obtained under aseptic conditions and carefully dissected from the underlying bone in each specimen [ 23].
Electron microscopy helped identify the novel virus, and tissue sections from biopsy and autopsy specimens showed the underlying SARS pathogenesis.
Test specimens included the underlying subchondral bone which prevents tissue swelling [ 17]; further details are available elsewhere [ 4, 18].
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