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They are highly diverse in terms of associated core promoter motifs, underlying sequence composition and patterns of transcription initiation.
The stability of mRNAs is regulated by signals within their sequences, but a systematic and predictive understanding of the underlying sequence rules remains elusive.
Different genetic strategies have yielded specific lessons about the roles of lncRNA transcription, the lncRNA transcript itself, and underlying sequence elements.
By an example we show that the stability result is the most general in the topic and that the assumptions on the underlying sequence spaces cannot be removed.
Topics include: statistical theory underlying sequence analysis and database searching; Markov chains and hidden Markov models; elements of Bayesian and likelihood inference; discrete data models; applied linear regression analysis; multivariate data decomposition methods (PCA, clustering); software tools for statistical computing.
Of practical consequence is that nucleic acid based tests in general may not yield a correct result because their performance depends on the underlying sequence information.
Here, as shown below, the grouping at 95% identity includes differences derived from PCR errors and from underlying sequence diversity in the microbial sample.
Neither enhancer nor promoter activity conservation can be explained purely by underlying sequence elements.
False negatives identified for Compara can primarily be ascribed to the underlying sequence input data.
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These methods advance our ability to develop broadly applicable genetic tools and to elucidate the underlying sequence-structure-function relationships that empower rational design of complex biomolecules.
Based on this evidence, we hypothesized that the overlap in miRNA and uAUG function may arise from underlying sequence-specific interactions.
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CEO of Professional Science Editing for Scientists @ prosciediting.com