Sentence examples for underlined nucleotides from inspiring English sources
During the PCR reaction, BamHI and SacI sites were introduced into the PCR amplified DNA fragment (underlined nucleotides in PCR primer sequences).
The nucleotide dispensation sequences for the G638A and A667G SNPs were G TC AGCAC and ACA TC AGAG, respectively (underlined nucleotides representing the negative control and bold nucleotides representing the polymorphic sites).
The gene encoding mature α-amylase SR74 was amplified using the forward primer 5′-CGCC CACGTGGCCGCACCGTTTAACGGCAC-3′ incorporating a PmlI site and the reverse primer 5′-TGGT TCTAGACAAGGCCATGCCACCAACCGTGG-3′ incorporating XbaI site (underlined nucleotides indicate the restriction endonuclease PmlI and XbaI sites).
> -wrap-foot> Unucleotidesucleotides represent cloning site We used A. nidulans A89 as the host strain for the construction of carboxypeptidase overexpressing strains because A. nidulans has lower proteases background levels than A. oryzae.
The underlined nucleotides represent the loop sequence.
The underlined nucleotides did not anneal with the cDNA but created a unique BamHI site and a unique HindIII site, respectively, as indicated by the italicised letters.
The underlined nucleotides are SmaI or XhoI sites.
REP sequences are denoted in 5´ to 3´ orientation as follows: conserved tetranucleotide in bold and italics, complementary (palindromic) nucleotides underlined, variable nucleotides (IUPAC code) in lower case.
Underlined nucleotide indicates residues changed from wild type PLN in generating mutation (See Figure 1C) Sigma 1R and SR β cDNA was purchased from Open Biosystems in the pDONR223 Gateway Entry Vector and then cloned into the Gateway pEF-Dest51 Vector (Invitrogen) which contains a V5 and 6His tags.
Nucleotides in bold are allele-specific and those underlined indicate nucleotides deliberately mismatched to the original gene sequences to increase primer specificity.
Primers for NFKBIA −826 C/T and −881 A/G were 5′-GGTCCTTAAGGTCCAATCG-3′ and 5′-GTTGTGGATACCTTGCA CTA-3′ (underlined, mismatched nucleotide), which amplify a fragment of 200 bps (28).
