Sentence examples for underlined in the reverse from inspiring English sources

Two BsmBI sites (underlined) in the reverse primer were included for cloning shRNA inserts.

Using these primers, the generated product has a NotI site at the 3' end (underlined in the reverse primer).

For each construct, a restriction site Xho1 (underlined) was included in the forward primer, and a myc Tag (bold), followed by a stop codon and restriction site XmaI (underlined) in the reverse primer.

The last 'C' was the starting 'C' of a Pst I restriction site (underlined in the S2 reverse-complementary sequence).

Restriction sites for BamHI and EcoRI are underlined in the forward and reverse primers, respectively.

The PCR product was digested with BamHI and XhoI (restriction sites underlined in the forward and second reverse primer, respectively) and subcloned into the pFASTBAC1 vector (Invitrogen) cut with the same restriction enzymes.

The passages in italics were underlined in the original.

This commitment has been underlined in the new Sustainable Development Goals.

The forward primer contains an EcoRI site (underlined) and the reverse primer contains a SmaI site (underlined).

The forward primer was 5'-AAGACATTTGAAAATCACGGCTCTGCAAACTCCTCCCCCTG-3' (mutations underlined), and the reverse primer was the forward primer's reverse-complement.

The forward primer contains a Spe I site at its 5' end (underlined) while the reverse primer has a Xho I site at its 5' end (underlined).