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In the forward primer, a BamHI restriction enzyme site was included (indicated in bold), while in the reverse primer a NotI (indicated in bold) restriction site and a stop codon (indicated by underline) was inserted.
The underline was MafB motif.
Dashed underline was the LRR peptide fragment used to estimate the Kd of dimerization and to raise the anti-Gm18RLK-1 antibody.
For the 57 non-survivors, classification improved using the model with PESI + WBC count; 1 subject (cell in underline) was reclassified down and 4 subjects (cells in bold) were reclassified up.
Similar(56)
Underline is blue.
The Australian community, he is keen to underline, is mainly happy with diversity.
PD: As long as the underline isn't, The Charles Manson Story.
So what I am really trying to underline is the importance of this choice.
Red is gRNA target, underline is insertion.
P values with underline are statistical significant.
The primers were H1-3.3, 5'- GGATCCUTGGTCTCATACAGAACTTA, where a BamH I site (underlined) was introduced; and Pu-3.1 5'- TCTAGAUTTGCCAAACCTACAGGTGGG, where an Xba I site (underlined) was introduced.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com