Exact(1)
P3: It is the gay community (note: underline added to denote participant emphasis) P1: Support exactly what he's saying.
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There is a little scroll wheel on the bottom right, can click on this "select wheel" to underline, add highlights, look up words in the dictionary, you select a line and it gives you the definition of every single word in that line.
The restriction sites of BamHI GGATCC, XhoI CTCGAG and SpeI ACTAGT (sequence in small letters underlined) were added to the end of the primer for conventional cloning.
Just in case the exclamation point doesn't get your attention, this phrase is often underlined for added emphasis.
For PP1α an additional K (underlined) was added to the N-terminus to aid coupling to carrier proteins.
Restriction enzymes sequences (underlined) were added to the primers to facilitate the subcloning (PstI in the forward primer, EcoRV in the reverse primer).
To construct pGL1163-2628, pGL2299-2628, pGL2299-2628, pGL2881-2628, and pGL3588-2628, a sequence (5'-AATTCGGTACC) containing the Acc65I restriction site (underlined) was added to the 5'-end of the oligonucleotide primers used for the PCR amplification.
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