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BrdU incorporates into the DNA of cells undergoing proliferation mainly in lymphoid tissues whereas CFSE labels ≥98% of resting and proliferating peripheral blood mononuclear cells [17], [18].
This allows cells to survive in a non-adherent state without undergoing proliferation.
This tissue was labelled by cumulative infusion, volumetrically quantified across time, and temporally analysed for the proportion of cells undergoing proliferation.
This suggests that at P6 Bergmann glia are in a state of active metabolism and growth, and some of them may potentially still be undergoing proliferation.
β-gal/LacZ staining was then performed and the presence of cells colocalized with BrdU (cells which are undergoing proliferation) and β-gal/LacZ (dedifferentiated cells) were determined.
Ki-67 staining (Fig. 5C) further showed that fewer TIP-1-positive cells were undergoing proliferation than the TIP-1-negative cells.
Similar(23)
LPS drove BGC-823 and SGC-7901 cell to undergo proliferation with a greatly increased DNA index (DI), which indicates the ratio of proliferating cells to resting cells.
Under an appropriate dosage, pro-angiogenic cytokines, such as vascular endothelial growth factor (VEGF) [6, 7], can up-regulate angiogenesis by signaling vascular endothelial cells to undergo proliferation, migration, and differentiation into new blood vessels.
We further analyzed these cells for their ability to undergo proliferation in response to VEGF.
During the process, chondrocytes undergo proliferation, hypertrophic differentiation, and apoptosis [1], each of which is regulated by distinct signals.
Myogenesis requires that muscle precursor cells (e.g. satellite cells), or myoblasts, undergo proliferation, followed by cell-cycle exit.
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