Although the master seed virus used for vaccine production undergoes only a few passages in cell culture, making the risk of mutation is very small if not negligible, field RV strains undergo passage in animals of various species and therefore can escape from the protection induced by oral vaccines.
When cultures reached confluence, they underwent passages by trypsinization and were seeded in different plates depending on the experiment.
To investigate visual function and optical quality in eyes with epiphora undergoing lacrimal passage intubation.
The CWD prion agent is undergoing serial passage naturally as the disease continues to emerge.
Tendon cells (undergoing one passage) were plated into 96-well microtiter plates.
These Theileria-infected cell lines did not undergo extensive passages (<30 passage) and were stored in liquid nitrogen until use.
Published studies have also shown that EBOV is not significantly evolving, despite undergoing extensive passaging in humans during the 2014 15 outbreaks (Hoenen et al., 2015) (Tong et al., 2015).
Further EB differentiation experiments were performed on cells after undergoing 8 passages, either automated or manual, in 24-well plates.
The cells used for chronic exposure were considered chronic after undergoing ten passages (cells trypsinized at 90% confluence and subcultured in another cultured flask).
As noted in previous studies of receptor-binding specificity (22, 24 ), the 1957 strains have undergone varying passage histories, but all have been passed at least once.
Two sets of three viruses (from a pig, human or mosquito) isolated during outbreaks in 1982 and 1985 in Kamphaeng Phet Province, Thailand, were selected because they had undergone limited passage in the laboratory (Table S1).
