PfMC-2TM proteins are most highly expressed in the mid-trophozoite stage and have been shown to undergo expression switching, suggesting that the Pfmc-2TM gene family plays a role in antigenic variation [ 18, 35].
However, distinguishing genes that undergo expression variation (EV) among all the genes analysed remains difficult.
However, in all cases, the selection of genes undergoing expression variation is associated with a stringency parameter.
Consequently, the normalization of gene expression data [11] and the development of methods to identify genes undergoing expression variation (EV) would represent an important step forward.
Another interesting finding was that a number of genes undergoing expression variation after LPS stimulation were involved in cellular lipid metabolism (Figure 2B).
Taken together, these data show that by decreasing EV occurrence, the number of genes considered to undergo expression variation can be increased for the analysis of macrophage activation by LPS.
Among the 300 macrophage genes undergoing expression variation upon LPS stimulation, the pathway analysis knowledge database has revealed that 178 gene products could be defined as "Top Bio Functions" thanks to their score (data not shown).
Likewise, a few duplicated gene pairs (GmIPT08 and GmIPT10; GmCKX05 and GmCKX06; GmCKX12 and GmCKX14) have undergone expression divergence in soybean [ 16].
In high throughput mode, it is possible to examine multiple synthesis products and undergo expression trials in 96-well plates as reviewed by Hughes and Ng [ 16].
In summary, genes that undergo expression alteration between wild and domesticated fibre do not do so randomly but, instead, they appear to have been shaped by human selection in a correlated fashion, forming clusters of genes displaying one to several of the possible expression patterns.
