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Additionally, expression of the neural precursor gene nestin was significantly down-regulated in all EDK and iPDK cells upon their differentiation, suggesting they did not undergo commitment to a neural fate (Figure 4A).
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Studies on gastrulation stage embryos suggest that the early temporal and spatial patterns of HNF-3 beta, MF-1 and HNF-3 alpha correlate with populations of cells undergoing commitment to different developmental fates.
Consistent with this, our data show greater specificity of Gfi1-35 acomparedcompared to the Scl+19 in day 4 EBs for cells undergoing commitment to the haematopoietic lineage.
METHODS: A total of 331 involuntarily hospitalized patients awaiting discharge under outpatient commitment were randomly assigned to be released or to undergo outpatient commitment.
In these conditions, ES cells undergo neural commitment through a "autocrine" induction mechanism, where FGF signalling plays a pivotal role, as it does in the embryo [17], [18].
A recent study has also provided insights into how HSCs undergo erythroid commitment.
Are actively dividing stem cells more likely to undergo lineage commitment than quiescent stem cells?
Cells also may undergo lineage commitment through different initial gene expression trajectories (Pina et al., 2012).
On this basis, we propose, the 'last cell standing hypothesis' in which PGCs are derived from the last cells in the embryo that are to undergo lineage commitment.
The distinctive transcriptional profiles of different hematopoietic compartments (see Kee, 2011 for an overview) imply significant changes in TF binding as cells undergo lineage commitment and differentiation.
However, Mbd3 mutant ES cells are viable but unable to silence genes expressed at preimplantation stages and undergo lineage commitment [ 37].
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