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Despite this lack of mechanistic clarity, it is worth noting that we have shown that the ROCK inhibitor Y-27632 also extends the number of passages that stem cell derived RPE can undergo and maintain their normal phenotype and function [ 122].
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We conclude that cdc13Δ exo1Δ pif1Δ mutants do not undergo senescence and maintain their telomeres for at least 11 passages (44 days).
Perhaps in response to these factors, some endothelial cells may undergo EndMT and maintain their mesenchymal phenotype indefinitely.
On the contrary, Burchelli's zebra fibroblasts, simultaneously transfected with h TERT- and h TERC-expressing constructs, did not undergo crisis and maintained a high proliferation rate during all the culture period.
This is similar to embryonic stem cells (ESCs), which are highly proliferative, undergo self-renewal and maintain few copies of mtDNA.
Exactly why DUXC and DUX4 (embedded in D4Z4) in particular should have repeatedly undergone duplications and maintained these copies is an unresolved question.
Our results indicated that BY-kinases underwent fast evolution and maintain a high conservation only for the residues directly involved in structure formation and catalytic activity.
Due to the adhesion, the adsorbed segments undergo a larger friction and maintain a longer residence time τ1 on the adhesive colloidal surface.
Like ESCs, iPSCs can undergo unlimited self-renewal and maintain the ability to differentiate into all three germ layers.
They can undergo unlimited self-renewal and maintain the embryonic stem cells (ESCs -like ability to diffESCs -likeinto abilityee germ layers.
Altogether these results showed that, at confluence, cells expressing high levels of Arl2 (MA+ and MdaA+) displayed a stronger growth arrest with increased contact inhibition whereas cells with reduced Arl2 content (MA- and MdaA-) did not seem to undergo growth arrest and maintained growth ability even at confluence.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com