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This always happened "under total darkness", he says.
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Seeds were cold-treated at 4°C for 2 days before being transferred to 22°C for germination and growth under 16/8 hr light/dark cycles, or in total darkness for dark-grown seedlings.
The defining characteristic was the presence of faint luminescence, which could be clearly seen with the naked eye when the colonies were examined under conditions of total darkness.
Later that night, despite thick clouds and drizzling rain, Antioch's men took our dinghy under the cover of total darkness and made their way behind the fringing reef and over toward Tuboa.
Next, 20-μL aliquot of mycelia suspension was transferred to a petri dish (P6) containing PDAD media, following incubation for 5 days under 298 K at total darkness.
Several spooky sequences — one unfolds in almost total darkness — will probably prove too unnerving for children under 8 or 9.
I am sat deep under the ground in a 2,000-year-old 2,000-year-old 2,000-year-old, in totalavarkness.
Total darkness.
In total darkness.
Total darkness enveloped me.
"Bioluminescence needs total darkness.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com