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Ethylene glycol bis[succinimidylsuccinate] (EGS) and m-Maleimidobenzoyl-N-hydroxysuccinimide ester (MBS) treatment were applied to plants growing in open chambers under the same media conditions as the closed chambers, but the protonemata were immobilized with 0.7% low melting point agarose.
However, the controls intrinsic to each experiment always permitted a comparison between strains grown under the same media conditions.
For the IGF-1 forward signalling experiments, HeLa and MEF cells were serum-deprived for 24 h, and then the IGF-1 R3 was added under the same media conditions, for different time points ranging from 1 to 24 h, as detailed in the Results section.
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The size of the rlsb-silenced plants varied between the lines, showing size reductions in overall shoot growth of approximately one-third to one-fourth by 6 to 8 weeks after germination, when compared to Col0 grown on the same media under the same sterile conditions.
Efforts were made to minimize oocyte damage by performing rapid denudation in bicarbonate buffered media and an appropriate gas atmosphere followed by morphological selection under a stereomicroscope by using the same media and environment.
The cells were thawed according to the manufacturer's recommendations and cultured in T75 flasks containing the same media and under the same conditions as BM-MSCs and AD-MSCs.
For the direct coculture analyses, we excluded genes that did not have at least twofold deviation from the mean in at least one sample and the method of Buess and colleagues [ 14] was used to normalize cocultures to appropriate monocultures performed in the same media and under identical conditions as described previously [ 5].
But it uses the same media to show it distributing aid and administering healthcare to people under its rule.
And most actors don't keep the same media schedule.
The yNhANT4-V5 yeastrainsins were cultured using the same media, rich glucose-containing media (YPD).
THP-1cells were also maintained in the same media.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com