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The presence of a preservative, under the same extraction conditions, resulted in a slight difference or no difference in the CGA and CFA extraction yields.
As controls, the pure fatty aldehydes proved to be quite stable under the same extraction and analysis procedures (Additional file 7: Figure S5).
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To achieve a compact structure, the two extraction systems are designed to share the same extraction channel.
The same extraction procedures were applied as those in the hexane extraction.
The same extraction procedure was also carried out using HPLC grade ethanol (Sigma Chemical Company) as the extraction solvent.
The same extraction procedure was repeated 3 times; the supernatant fluids from 3 extractions were combined and the volume condensed under reduced pressure at 50°C.
DNA extractions of these samples were conducted using the same extraction protocol as for aDNA samples.
The method uses the same extraction procedure of compendium pharmacopoeias.
The authors used the same extraction procedure and focused on the same isotopes as Koons [ 67].
As the results in Stockholm and Uppsala originated from the same extractions, they were pooled.
All strains were cultured in triplicates under the same conditions, and the extraction of each sample was performed in parallel.
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