In cells that were cultured under conditions allowing optimal expression of photosynthetic pigments the development of an intracytoplasmic membrane system was detected by transmission electron microscopy of ultrathin sections (Figure 2D).
The soluble fraction is indeed a mixture of covalent (fully active) and non-covalent mBCO forms: under optimal expression conditions, nearly 55% of the soluble recombinant mBCO is produced as covalently FAD bound form.
Interestingly, under optimal expression conditions, nearly 55% of the soluble recombinant BCO is produced as covalently FAD bound form, whereas the protein containing non-covalently bound FAD is preferentially accumulated in insoluble inclusion bodies.
This might occur when specific genome-wide loci are under strong selection for optimal expression levels between the sexes, with dosage compensation of Z-linked genes allowing the fine tuning of sex-specific dose.
Furthermore, expression profiling can facilitate the design of optimal expression plasmids for use in specific cancers.
Strains grown under full induction of LacZ and fixed concentrations of lactose evolved to express the predicted optimal levels of the enzyme, highlighting the fitness advantage obtained by optimal expression [ 6].
We have chosen a minigenome-based functional assay, the VLP infectivity assay (described under Materials and Methods), to determine optimal expression plasmid ratios for efficient VLP formation based on reporter gene readouts.
Under the optimal diet, 240 probe sets representing 220 genes showed a higher expression in SR than in C flies.
For optimal expression in Lactococcus lactis [27], residues 2 14 and 309 315 were removed by PCR, including the His-tag, and the resulting genes were cloned into the expression vector pNZ8048 under the control of a nisin A-inducible promoter [28].
The maximum DH (35.13%) was obtained under the optimal conditions.
