These results imply that the pyc of C. glutamicum is under the highest selective constraint, followed by maeB of E. coli, maeA of E. coli, and pyc from B. subtilis.
Further investigation of four enzymes that catalyze the conversion of pyruvate to malic acid and OAA and eventually to succinate demonstrate that pyc of C. glutamicum is under the highest selective constraint, followed by maeB of E. coli, maeA of E. coli, and pyc from B. subtilis.
This evolutionary study on Lagomorpha TRIM5α gene shows a remarkable differentiation in the PRYSPRY v1 region suggesting that this gene has evolved under a high selective pressure within the Lagomorpha order.
On a genetic basis, gene conversion should help to preserve the sequence of the proximal promoter region under high selective constraints, which only occur between highly similar sequences, and always at >92%.
All together, our data suggest that this set of residues is under a high selective pressure to keep a suitable redox environment around the heme prosthetic groups.
However, looking at the pS values among many of the pairwise SMP comparisons it is apparent that many of the SMP sequences are maintained under high selective pressure similar to other sequences (Table 3).
In addition, miRNAs highly expressed are reported to be under a high selective pressure to maintain their sequence unaltered in comparison to lowly expressed miRNAs that are rapidly evolving with frequent mutations [ 44].
Finally, examination of mean dS/dN values for PSMR pairs, ykkC/ ykkD (1.7) and yvdR/ yvdS (2.0), revealed that each pair is maintained under moderately high selective pressures in comparison to all other PSMR members (Additional file 2).
There is currently accumulating evidence that the distribution of cellular resources is under high selective pressure, especially in fast growing prokaryotes.
A large fraction of genes was found to be under high selective constraint, as most of the expressed genes appeared to be involved in developmental gene regulation.
