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In addition, the stability of the extract under gastrointestinal conditions was evaluated by HPLC DAD-ESI MS/MS.
The composition of the extract under the predicted optimum conditions (i.e., 109 bar, 56 °C, 0.86 mL min−1, and 61 min) was about 69% triacetin accompanied by only 30% diacetin and no detectable monoacetin.
Insecticides are stable in the extract under these conditions.
He found that insulin precipitated from the extract under mildly acidic conditions.
The following compounds were identified in the extract obtained under optimum conditions: monogalloyl glucose, digalloyl glucose, -gallic acid, -epicatechin, -catechin, ellagic acid and quercetin 3-O-rhamnoside.
Table 5 displays the factor loadings of the 65 items under the extracted factors.
The relative abundance of histone H2B isoforms and their acetylated forms was calculated based on the area under the extracted ion chromatograms from the limited mass-range MS1 scan for all of the detectable charge states of each H2B peptide.
To determine the relative abundances of each modified form of the peptide, the areas under the extracted ion chromatogram for each peptide in all of its charge states and modified forms were integrated and summed.
Under this condition, the extract reduced the yield of the acyclovir-sensitive HSV-2 with an IC50 value of 1.46 μg/ml (95% CI: 1.07 to 1.91).
Under the optimal conditions, the extract yield was 2.38%, which is comparable to the extraction of other polysaccharides (0.69 3.66%).
The organic solvent was removed by placing the extract under nitrogen stream.
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