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Exact(6)
This event occurred independently in all populations under temperature selection, although the haploid variants arose at different times and swept their respective populations over different time intervals.
This collection of variants was used to found six replicate populations that evolved under glucose-sufficient, nitrogen-limiting conditions: three under temperature selection and three under ethanol selection.
Under temperature selection, adaptive changes in zymolyase and Micafungin resistance could be expected inasmuch as cell wall integrity has been shown to play a key role in thermal stress response [ 63].
In clones that arose under temperature selection, thermal tolerance was significantly greater than that of isolates in the founder population and of the parental F1 strain, a result likely due in part to segregational loss of the temperature-sensitive S. uvarum genome, resulting in S. cerevisiae haploids.
As prior experiments in S. cerevisiae had shown that thermal tolerance confers cross-protection against other types of stress [ 37, 43], we further hypothesized that hybrids evolving under temperature selection would not only become more thermal tolerant than their ancestors but also exhibit greater ethanol tolerance, and vice versa.
Acquisition of thermotolerance in diauxic and stationary phase yeast has also been linked to accumulation of the disaccharide trehalose [ 64, 65], however, we uncovered no evidence that trehalose hyper-accumulates in heat-shock resistant clones that arose under temperature selection (data not shown).
Similar(54)
Following 500 generations of temperature selection under nitrogen limitation, populations in vessels A and B appeared to be karyotypically monomorphic, whereas the population in vessel C was polymorphic (e.g., Figure 6B, lane 20), albeit with a numerically dominant clone.
Thus, under this scenario, the modern-day NT-MAT correlation is primarily a result of biogeographic history, and secondarily altered by effects from exaptive temperature selection on toothed lineages.
For example, it is also possible that Idh-1 is linked to another locus which is under temperature-driven selection, and that the allele frequencies observed in nature reflect this linkage disequilibrium [ 5, 27].
Pupal survival rate under temperature shock increased through time in the selection line, as predicted under this hypothesis.
Temperature-responsive smRNAs were identified predominantly under temperature stress conditions.
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