To detect CsPRR clones other than TOC1, a full length CsTOC1/PRR1 clone was used as probe on two replicate membranes, one under high and the other under low stringency hybridization and washing conditions.
All these were HPV negative with both the HPV 6/16/18 screening probe mixture under low stringency and the HPV 16 probe under high stringency.
A basic social teaching of Islam is the encouragement of marriage, and the Qurʾān regards celibacy definitely as something exceptional to be resorted to only under economic stringency.
For example, Hashimoto and colleagues isolated it as a PACAP receptor from a rat brain cDNA library by cross-hybridization with rat VIP receptor cDNA under low stringency conditions [21].
Under higher stringency conditions (a lower buffer concentration, in this case, 0.5 × SSC), the reduced level of hybridization can be observed for both targets, but for the less complementary oligonucleotides, it was somewhat much stronger (Fig. 2b).
The latter was a single, one-step reaction under high stringency conditions, using simultaneously 2 pairs of primers (1 pair for C. sinensis product size 612 bp, and 1 pair for O. viverrini product size 1357 bp).
All of the above interactions are robust and deemed strong, as none of the negative controls reveal positive interactions under low stringency and many bona fide positive interactions may not survive as low as 1 mM 3-AT concentration under the same experimental conditions (data not shown).
Total RNA was isolated from three developmental stages, pretreated with DNase I, and hybridization was performed under high stringency conditions.
Further Western blotting of the FLAG-PARP1 immunoprecipitates confirmed the presence of lamins A and C (Figure 2B, lane 2, αLmnA/C; n>4) under high stringency conditions, as expected [51], [52]; and also revealed low levels of the nuclear membrane protein emerin (Figure 2B, lane 2, αEm; n>4), with higher signals under low stringency conditions (not shown).
