201B7 and 253G1 hiPSCs were cultured on Matrigel-coated dishes with mTeSR1 (Stem Cell Technologies, Vancouver, Canada) under standard cell culture conditions (37 °C, 5% CO2).
Samples were placed inside for the ischemic period, followed by a reperfusion stage under standard cell culture conditions.
Normal human dermal fibroblasts (NHDFs) (Lonza, Walkersville, MD) were cultured under standard cell culture conditions in Dulbecco's modified Eagle medium (DMEM) (Nacalai Tesque, Kyoto, Japan) containing 10% foetal bovine serum (FBS).
Human cardiac myocytes (ScienCell, Carlsbad, CA) were cultured under standard cell culture conditions in cardiac myocyte medium with cardiac myocyte growth supplement and penicillin/streptomycin solution containing 5% FBS (ScienCell).
Then, supernatants were discarded and fresh medium with CCK-8 (Cell Counting Kit-8) solution (Dojindo, Kumamoto, Japan) was added and incubated for 2 h under standard cell culture conditions (37 °C, 5% CO2).
To assess the impact of the culture mode on the cells' sensitivity to chemotherapeutics, HSPCs were cultured together with MSCs for 5 days under standard cell culture conditions (2D) or within the macroporous 3D scaffolds as described above under static and dynamic conditions in the presence or absence of 5 µM 5-fluorouracil (Merck, Darmstadt, Germany).
Cells were grown under standard cell culture conditions in 5 % CO2 at 37 °C to reach confluence of 50%% to 60%% before subjected to any further treatment.
Cells were then allowed to adhere on the substrates under standard cell culture conditions (37 °C temperature, 5% CO2and 95% humidified air) for 4 h.
Both dental cell types expressed the pro-apoptotic marker BAX and the anti-apoptosis marker BCL2 under standard cell culture conditions (Figure 3B).
Separately, 30 μg of FITC-labeled anti-CA125 MAb was added to NIH OVCAR-3 cells growNIH OVCAR-3lips and allowed to incellse under standard cell culture conditions over a period of 48 h to study uptake in live cells.
Under standard cell culture conditions, fiber binding lasted for at least 12 hr (Figure S2).
