Exact(3)
When integrins were inhibited in nonsphere glioma cells, the TGF- β pathway was strongly impaired, whereas no such effect was observed in glioma cells cultured under sphere conditions.
Interestingly, strongest enrichment of up-regulated genes was seen for lysosome related genes (P = 2 × 10-18) and genes involved in oxidative phosphorylation (P = 3 × 10-14) indicating a requirement of lysosomal activity and energy generation under sphere forming conditions.
In the mouse glioma model systems used here, we noticed extensive cell death following integrin inhibition in all four mouse glioma cell lines tested when cultured as adherent cells, that is, under standard conditions, whereas no such effect became apparent when the same cells were cultured under sphere conditions that we had explored as a possible in vitro model of stemness.
Similar(56)
This cracking phenomenon and mechanism are analogous to the ring/cone crack formation of brittle materials under sphere-sliding contact.
During a sliding test performed under sphere-on-plane contact conditions, the contact area between the antagonists is the minimum at the beginning of the test.
One limitation to our approach is the loss of potentially important antigens from the cell populations that were unable to proliferate under sphere-forming conditions.
In addition, the SP and the main tumour cell population (MP) were sorted by FACS for RNA extraction to profile gene expression, and for culturing under sphere-forming conditions.
UPPER WEST SIDE Stars of Jazz and Sky Vie for Attention at Hayden Jazz stars are coexisting with galactic ones at "Starry Nights: Fridays Under the Sphere" under the Hayden Sphere at the new Rose Center for Earth and Space.
"Starry Nights: Fridays Under the Sphere," music tonight from 5 45 to 8, with Mark Whitfield Quartet.
"Starry Nights: Fridays Under the Sphere," music tonight from 5 45 and 7 15, with the Mark Whitfield Quartet.
"Starry Nights Under the Sphere," with Chico Hamilton, jazz drummer, and his quintet, Euphoria.
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