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We obtained nine timed pregnant Wistar rats (250 300 g; Charles River Laboratories, St. Constant, Québec, Canada) on gestational day (GD) 14 and housed them in single plastic cages with a bedding of sawdust under regulated temperature (21 ± 2°C), relative humidity (50 ± 10%), and a 12-hr light/dark cycle.
Timed-pregnant Long-Evans rats (body weight ~ 230 g; Charles River Laboratories, Portage, MI) were obtained on gestational day (GD) 3 (day of insemination is GD0) and were housed individually in standard polycarbonate plastic cages containing heat-treated pine shavings as bedding and maintained under regulated temperature (21 ± 2°C), relative humidity (50 ± 10%), and a 12-hr light/dark cycle.
We obtained timed pregnant Long-Evans Hooded rats (~ 230 g; Charles River Laboratories, Portage, OR) on gestational day 3 (Gday day of insemination = GD0) and housed them under regulated temperature (21 ± 2°C), relative humidity (50 ± 10%), and a 12-hr light/dark cycle.
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The powdered material was extracted with ethanol (Merck Chemicals Pty Ltd Wadevillele, South Africa) under pressure (100 bar) and regulated temperature of 50°C in a BUCHI Speed Extractor, E-916 (BUCHI Labortechnik AG, Switzerland).
Mice were housed in groups of five per cage with free access to food and water, under controlled light/dark cycles, in facilities with regulated temperature and humidity.
The idea is to immerse them in a regulated temperature that isn't as hot as boiling water, with the intention of cooking them evenly throughout.
Hilotherapy is the application of cold compression at a regulated temperature through a face mask.
and in a room with regulated temperature (range 22 25°C).
Britain's private rented sector is under-regulated, but needs regulation that lets the market breathe.
The forced-flow CVD was carried out by heating according to a temperature program under regulated vacuum pressure.
The livestock industry is already under-regulated, Hunt added.
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