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This operation was repeated seven times at room temperature under reduced light to avoid oxidation.
Synthesis of the conjugate was performed under reduced light and under nitrogen to minimize auto-oxidation.
Because of the photooxidation of malachite green, tubes were covered with foil and the experiments were performed under reduced light.
All extraction procedures were carried out under reduced light in order to prevent oxidation of the compounds.
Cells were loaded with the Ca2+-sensitive dye Fura-2 AM (1.5 μM), by incubation (30 min; 37 °C) under reduced light in DMEM growth medium.
Optimal growth was observed under reduced light intensity (below 60μEE), when using standard fluorescent bulbs augmented with 18,000 K fluorescent bulbs (e.g. Aqua-Glo, Rolf C. Hagen Corp., Mansfield, MA).
Similar(53)
All handling of retinoids was performed under yellow reduced light.
All the steps described were conducted under a reduced light level to prevent additional DNA damage.
Catfishes are generally most active at night or under conditions of reduced light intensities.
Slides were immersed in the staining solution under conditions of reduced light for 45 minutes and washed in deionised water.
Dynamic changes in relative tiller number (Fig. 2) suggest that varietal differences in tillering ability and biomass production under SF are largely attributable to reduced light interception and concurrent lowering of photoassimilation.
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