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In order to investigate the decontamination potential of the biodiesel reaction under our defined conditions we performed alkaline methanolysis on MBM spiked with 5% 263K scrapie infected brain (MBM sc).

We first established reference values for RT, MT (= TBRT) and BF under our defined experimental conditions.

NGS showed a p.V600E mutation with a 3% allele frequency being under our defined threshold.

To further characterize the MP cell population derived and expanded under our defined culture conditions, we performed transcriptome analysis by RNA sequencing (RNA-seq).

Similar(56)

While our studies highlighted 18S as the most stable of endogenous controls under all four of our defined experimental conditions, it should be noted that there are concerns with using rRNA as a control which may not be influenced by degradation machinery in a matter similar to mRNA [15], [37].

Given the induction of Wnt and Lgr5 after injury, and the existence of pancreas progenitors in the ductal tree (Criscimanna et al, 2011; Furuyama et al, 2011), we reasoned that adult pancreas progenitors could be expanded from the duct cell compartment under our previously defined gut and stomach organoid culture conditions (Sato et al, 2009; Barker et al, 2010).

Next, we examined whether hiPSCs were generated from the reprogramming step under our feeder-free, defined culture conditions.

I operationalize this definition somewhat differently from in previous work: Where the coalescent rate is known under our model, I define Ne = 1/λ2.

Interestingly, NGS showed a 2% allele frequency for p.V600E in this case being under the cutoff defined for our study (case 30).

Publishing findings in scientific literature is critical to sustaining the goals defined under our MEPI programme.

We incorporate human-related cell ontological terms into our integrated pages to provide formal names of cell types under the defined vocabulary.

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