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An average 9.68 g·L−1HLC III production was attained in the validation experiment under optimized condition, which was 80% higher than the yield of 5.36 g·L−1 before optimization.
Under optimized condition maximum xylanase activity found 260 U/ml.
PF with high Mw of 39,100 g/mol is obtained in 20 min under optimized condition.
Under optimized condition maximum xylanase activity observed 100.23 IU/ml, which was 9% higher than predicted by the model.
Under optimized condition, the calibration curves were linear (r2 > 0.997) in the range of 0.5 200 μg L−1.
The maximum loading (33.69%) was achieved under optimized condition (pH: 1.8, time: 3.54 (h) and drug/silica ratio: 1.7).
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The isolated yields under optimized conditions are shown in Fig. 3b.
Two TPSs, TPStp and TPS70c, were modified under optimized conditions.
The percentage removal of MB approached 67.21% under optimized conditions.
Under optimized conditions xylanase yield was 45.9 U/gds.
Under optimized conditions, 5 g/L norleucine was biosynthesized.
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