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Patients should be under observation using periodical CT, bone scintigraphy, or PET periodically.
Study physicians had participated in training sessions and actual examination of patients under observation using a formal certification process.
An apoptotic index was determined as the percentage of apoptotic cells, at least 200 counted cells under observation using an inverted microscope.
Age group-specific pneumococcal bacteremia incidence rates were calculated by dividing the number of cases by person-years under observation using corresponding population estimates from Thailand's National Economic and Social Development Board.
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Anatomical structures were identified under direct observation using the atlas and nomenclature of Paxinos and Watson [68].
Briefly, microdissection was performed under direct observation using an inverted microscope and a sterile needle.
Worms were placed on 2% agarose pads in M9, anaesthetized with levamisole (3 5 mM; Sigma) and mounted under a coverslip for observation using a Leica DM-RA microscope equipped with DIC (Normarski) optics and standard epifluorescence with filters appropriate for detection of YFP, CFP/SYTO41, SYTO59 or GFP.
For microscopic evaluation, worms were placed on 3% agarose pads and anaesthetized in 10 μl M9 containing 5 mM levamisole (Sigma-Aldrich, St Louis, MO) and mounted under a coverslip for observation using a Leica DM-RA microscope equipped with DIC (Nomarski) optics.
You need to be under observation when using morphine, because it has the potential for side effects.
Supernatants from macrophages under observation were used to determine NO as NO2 by standard Griess reagent assay.
The major tool of structuralist psychology was introspection (a careful set of observations made under controlled conditions by trained observers using a stringently defined descriptive vocabulary).
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