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The eluate (1,200 μL) was evaporated under nitrogen using a 96-well-plate evaporator system.
Catalytic degradation of high-density polyethylene (HDPE) was carried out under nitrogen using a laboratory fluidised bed reactor operating at 360 °C with catalyst to polymer feed ratio of 2 1 and at 450 °C with catalyst to polymer feed ratio of 6 1 under atmospheric pressure.
The [14C] labelled cells were initially resuspended in CH3OH/0.3% NaCl (2 ml, 100∶10, v/v) and mixed with 1 ml of petroleum ether (60 80°C) for 15 min. The upper petroleum ether layer was removed and a further 1 ml of petroleum ether added, followed by further mixing for 15 min. The petroleum ether extracts were combined and evaporated under nitrogen using a heating block.
DSC was performed with a TA-DSC 2010 apparatus (TA Instruments, New Castle, DE, USA) under nitrogen using aluminum sample pans.
7a All chemicals were used as received from the supplier and where required reactions were performed under nitrogen using Schlenk techniques.
The samples destined for GC/MS analysis were redried under vacuum desiccation for a minimum of 24 hrs prior to being derivatized under nitrogen using bis trimethylsilyl) triflouroacetamide (BSTFA).
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The samples destined for GC/MS analysis were re-dried under vacuum desiccation before derivatization under dried nitrogen using bistrimethyl-silyl-triflouroacetamide (BSTFA).
With GC/MS, the samples were further dried under vacuum desiccation for an entire day and derivatized under dried nitrogen using bistrimethyl-silyl-trifluoroacetamide (BSTFA).
The samples destined for GC/MS analysis were dried under vacuum desiccation for a minimum of 24 h and then derivatized under dried nitrogen using bistrimethyl-silyl-triflouroacetamide (BSTFA).
All samples were first analyzed under pure nitrogen using the temperature protocol of the phase 1 method before proceeding under air with phase 2. The phase 1 method was performed under 70 mL min−1 of nitrogen flow through the combined 25 mL min−1 of protective gas and 45 mL min−1 of purge gas.
Frozen A. niger mycelia were ground to a powder under liquid nitrogen using a mortar and pestle.
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