Sentence examples for under mild vacuum from inspiring English sources

The remaining acetonitrile was evaporated under mild vacuum conditions for 30 min using a rotary evaporator.

The precipitation is slowly dried overnight under mild vacuum and then slowly re-dispersed under mild agitation in pH 7.2 0.1 mol/L PBS with the final concentration of 1 μmol/L.

For AFM imaging, the fibril suspension was diluted 100-fold and 10 µl of the diluted sample were deposited on mica and then dried under mild vacuum.

Briefly, 600 µl of 10% PLLA/THF solution was cast under mild vacuum into an assembled sugar template by bound sugar spheres approximately 250 425 µm in diameter.

In the second procedure, a 2 µl aliquot was diluted 400-fold and 10 µl of the diluted solution were deposited on mica and then dried under mild vacuum.

All washes were performed under mild vacuum.

Labeled eluent collection tubes (Costar cluster tubes #4413; Corning Inc., Corning, NY, USA) were placed under SPE columns and samples were eluted under very mild vacuum with 300 μL of 2%NH4OH/90%% methanol.

The oil and n-hexane solution was then evaporated under a mild vacuum with a rotary evaporator.

Two microliters of cell supernatant or cell lysate protein was applied to ABS-Tween moImmobilon-Ny+membraneMillipore(Millipore, Billerica, MA, USA) under a mild vacuum in dot-blot apparatus (Minifold, Sigma-Aldrich).

The membranes were washed twice in 200 ml of ABS-Tween for 30 min each time under a mild vacuum, blocked in TBS-skimmed-milk solution for 1 h at room temperature and incubated in TTM-primary antibody solution overnight at 4 °C.

A mild vacuum pressure further increases blood flow to the hand.