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The skin scraping is then evaluated for the presence of hyphae (fungus strands) under microscopy, using potassium hydroxide (KOH) wet-mount preparation.
DNA was extracted from 10 μm sections stained with 0.1% methylene blue by manual microdissection under microscopy using 20-gauge needles and proteinase K solution as described previously [ 29].
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The concentration of the stock suspension was estimated by comparing the optical density at 600 nm (OD600) with McFarland standards, which was further confirmed by counting the cells under light microscopy, using a Neubauer chamber under phase contrast.
Motor cortical counts were normalized to the measured volume due to the lack of clear boundaries for regional outlining in the cortex.> Slides were examined under brightfield microscopy using a Zeiss Axioskop 2 plus microscope under a 10× objective lens.
Permanent slides were investigated and photographed under light microscopy using an Olympus BX43 with Olympus DP11 digital camera attached.
Transfected cells were identified under epifluorescent microscopy using green fluorescence protein (GFP) as a reporter gene.
The blue fluorescence was detected under fluorescence microscopy using a DAPI filter.
The number of branches and lengths of the microvessels were monitored on a daily basis, by examining the cultures under brightfield microscopy using non-phase inverted optics (Supplementary Fig S1).
The ventral prostate tissue was analyzed under light microscopy, using stereological, morphometrical and immunohistochemical techniques.
Ticks were all identified under light microscopy using morphological identification keys [ 20, 21].
Measures were done under light microscopy using an immersion objective (1000X).
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