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Periodically, organoids were removed, under microscopic visualization, for propagation into new culture flasks or phenotypic and molecular analysis.
In separate cultures, individual cell colonies were detached by local application of trypsin under microscopic visualization.
Each colony was trypsinized locally under microscopic visualization in order to detach stem cell colonies and detached cells were collected.
Precise microdissection from archival paraffin-embedded tissues was performed under microscopic visualization using a micromanipulator, as described previously.
These procedures were performed under microscopic visualization following removal of debris and cleaning of the external auditory canal.
After T12 L1 laminectomy under microscopic visualization, a midline durotomy was performed and a well-defined, encapsulated, round lesion was found in the conus medullaris region.
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We laser captured these areas by separate under direct microscopic visualization by melting of the selected regions onto a thermoplastic film mounted on optically transparent LCM caps using the Arcturus XP equipment (Arcturus Engineering, Mountain View, CA, USA).
LCM was performed under direct microscopic visualization by melting thermoplastic films mounted on optically transparent LCM caps (Arcturus) on selected populations of cells; β-cell rich tissue was identified by intrinsic fluorescence [18].
All factors were quantified under direct microscopic visualization.
Individual tumor cells were aspirated under direct microscopic visualization (Axio Observer A1, Zeiss, Thornwood, NY, USA).
This is a technology for procuring specific tissue cell subpopulations under direct microscopic visualization of a standard stained frozen or fixed tissue section on a glass microscope slide.
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