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Therefore, we also tested the PEG10 promoter under low serum concentrations (0.2% FCS).
The activity of the promoter constructs were either unchanged or slightly up regulated under low serum concentrations with the exception of PEG10-prom−1007.
Therefore, we analysed the promoter activity under low serum conditions by incubating the cells in the presence of 0.2% FCS for 16 hours.
This association was diminished again under low serum conditions (data not shown).
This indicates that Id3-induced decrease in cell number under low serum is caspase dependent.
Interestingly, SPP86 appears to enhance the proliferation of 8505C cells which express constitutively activated BRAFV600E under low serum conditions.
Similar(34)
Thus, wild type and TS-clathrin heavy chain cDNA transfected cells were selected under low-serum and low-temperature conditions for growth, because only those expressing functional clathrin could survive.
Seventeen of 35 original tumors could be cultured under low-serum conditions, the remaining failing to grow probably because of the reduced number of collected cells.
This discrepancy might be explained by the culture system we are using in which we culture cells under low-serum conditions, having low basal Akt activity.
Cells were incubated in the presence of EREG under low-serum conditions, with or without inhibitory antibodies directed against either ErbB1 (Erbitux®) or ErbB2 (Herceptin®).
Tissue inhibitor of metalloproteinase-3 has been reported to stimulate the proliferation of growth-retarded, nontransformed cells maintained under low-serum conditions (Yang and Hawkes, 1992).
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