Here, we present a system for single-cell lysis under light microscopy observation, followed by rapid uptake of the cell lysate.
Site observations on coal sections and observations under light microscope and scanning electron microscopy (SEM) were combined to understand the fracture characteristics of the coal seam, and the spatial geological model of fracture development was built.
The cystidia are generally smooth, but when dried material is mounted in potassium hydroxide for observation under light microscopy, an amorphous substance apparently holds spores and debris around the neck or apex, making them appear encrusted.
Morphological evaluation and determination of chitin were carried out by observation under light microscope and calcofluor white staining, respectively.
Observation under light microscopy will detect intact eosinophils, whereas degranulated eosinophils, which have released MBP, will be more difficult to quantify.
Apoptotic morphological changes were detected with Giemsa (Sigma-Aldrich) (3% in 15 m M phosphate buffer pH 6.8) and Hoechst 33342 (Dojindo Laboratories, Kumamoto, Japan) staining followed by observation under light and fluorescence microscopes, respectively.
A blood smear was also made extemporaneously with fresh or whole blood in EDTA, air-dried, stained using the May Grünwald Giemsa method and stored at room temperature before observation under light microscopy to search for intra-erythocytic forms of piroplasms.
nZnO particles were also not detected under polarized light microscope observation.
This characteristic permits, under polarized light, the observation of thin collagen fibers with weak birefringence in green color.
While aggregates were usually seen in the cell body or proximal dendrites, they were found around the nuclei (peri-nuclear), or even appeared to exist within the nuclei under a light microscopic observation (Fig. 7c, arrow).
In this study, the anticancer effect of ACBP-L in human gastric cancer is demonstrated by suppression of cell line MGC-803 proliferation in vitro by MTT assay and morphological observations under light or electron microscope.
