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As discussed above under constraints, structures with fewer interconnections are under less constraint and thus, are more variable.
However, Plasmodium cell invasion proteins and CCCH fingers are also under less constraint.
However, unique aspects of Plasmodium's lifestyle influence the constraint-gene group relationship as genes involved in the specialized cell invasion process are also under less constraint.
Overall, the behavior of Plasmodium's protein coding regions confirms observations from other eukaryotes in that transcription factors are under less constraint than proteins involved in core processes.
In Cuscuta nitida, the RNA-binding domain is evolving under less constraint than in other Cuscuta species and outgroups where multiple group IIA introns spliced by matK are still present.
If this is true, then copy number in HT genes may be under less constraint than in core genes.
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The mean ENC values of genes under stronger selective constraint should be lower than those of genes under less selective constraint (Akashi 1994; Stoletzki and Eyre-Walker 2007).
A proportion of this bias may be explained by selective constraint, in that sites that are polymorphic within GH1 may be under less selective constraint and thus more free to exhibit sequence divergence among paralogs.
Therefore, UP genes – which are frequently under less selective constraint – may produce longer alignments due to the introduction of gaps.
The high accumulation rate of mutations can be interpreted partially as genes being under less selective constraint.
This finding is consistent with the conclusion that non-coding regions such as UTR regions are under less functional constraint than coding regions.
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