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For complementation of strains WRG6 and WRG23 a low-copy plasmid harboring phoPQ under its natural promoter was generated.
This result is consistent with the GneM712T/M712T knock-in construction, where the mutated Gne gene remains under its natural promoter.
We made both extracellularly and intracellularly YFP-tagged versions of LNKN-1 expressed under its natural regulatory specific promoter.
Previous data showed DynA-GFP localization to the membrane and sites of septation when expressed under its natural promoter in B. subtilis.
A similar location for the Tsr2-3xGFP fusion protein (expressed from a CEN plasmid under its natural promoter and terminator regions) was observed in a Tsr2-depleted strain.
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Although it is vital to examine in vitro an RNA that contains the motifs of physiological relevance, and to refold it and probe it under conditions that resemble its natural environment as closely as possible, many larger RNAs exist as a mixture of different structures in vitro (which may reflect the situation in vivo) [ 22].
A major goal of this study was to discover the enzymes A. nidulans secretes to digest sorghum stover while growing under conditions mimicking its natural habitat.
Chrysosplenium americanum (Schwein x Hooker; Saxifragaceae), was collected from St. Anicet, Québec, and was maintained in the greenhouse under conditions simulating its natural habitat regarding light intensity, temperature and humidity.
The spiritual nexus of history "is that of life itself insofar as life produces connectedness under the conditions of its natural environment" (Dilthey 2002, 280).
Briefly, a retrotransposition competent LINE-1 element (LRE3) was cloned into an expression plasmid, under the control of its natural promoter.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com