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PCR was performed on a 7500 Fast Real-Time PCR System (Applied Biosystems), under following condition 94°C for 15 s, 55°C for 30 s, and 72°C for 30 s, total 40 cycles.
After an initial denaturation 94°C for 5 min, 30 cycles of amplification were performed under following condition: 94°C for 30 seconds, 56°C for 45 seconds, and 72°C for 40 seconds.
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Sequence PCR was run under following conditions; 94°C for 3 min, followed by 50 cycles of 96°C for 30 sec, 50°C for 10 sec and 60°C for 3 min each.
Simulations were done under following conditions: 16-QAM modulation, γ=−12 dB, N=1000 samples, and N s =4 sps.
Jig tests were performed under following conditions: pulsation amplitude of 4 mm and feed rate of 2.5 kg/min.
The diffractograms were recorded under following conditions: voltage 40 kV, 35 mA, angular range 5 and fixed divergence slit change.
The GC separation was performed under following conditions: oven temperature programmed to increase from 60 °C (5 min) to 180 °C (5 min) at a heating rate of 4 °C/min.
The patient has an increased risk of primary headache under following conditions; Neither comorbidity nor neck low back pain is present.
The culture was allowed to grow under following conditions: 0.5 vvm air, 0.33 bar pressure, 100 RPM agitation and 28 °C temperature.
Shaking table test was performed under following conditions: shake amplitude 5 mm, shake frequency 180 cycle/min, longitudinal slope 3 degree and feed rate 200 g/min.
The sample was loaded via electrokinetic injection (10 s, 25 kV) under following conditions: column 60 cm × 75 μm; background electrolyte: benzimidazole 20 mmol/L + acetic acid 40 mmol/L; UV 230 nm, separation voltage 25 kV, 20 °C.
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CEO of Professional Science Editing for Scientists @ prosciediting.com