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Approximately 20% of oocytes routinely retrieved following hormone stimulation are classified as immature (termed germinal vesicle (GV) or metaphase I (MI) stage); these oocytes are discarded due to their reduced potential for embryo development under current culture conditions [1].
We found that hSKPs quickly became senescent under current culture condition, which was at least partially responsible for their loss of self-renewal ability.
Under current culture conditions, oocytes for pig SCNT embryos were allowed to mature in vitro, and the reconstructed embryos were cultured in fatty-acid-free medium.
However, despite being promising stem cell reservoir for dental tissue regeneration, PDLCs and DPCs inevitably undergo replicative senescence under current culture conditions, resulting in cellular phenotypic changes [ 7– 9].
Similar results have been reported previously in natural populations of sulfur-oxidizing acidophilic bacteria (Knickerbocker et al. 2000), indicating that A. thiooxidans has a competitive advantage over A. ferrooxidans under current culture conditions.
Although UGTs were not detected in HBEC-KT, H358, and BEAS-2B under current culture conditions, the contribution of UGTs in human lung cells to B[ a]P-7,8-catechol conjugation may be affected by the induction of UGTs.
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Interestingly, the articular cartilage under the current culture conditions did not lose its IGF-I responsiveness.
Interestingly, under the current culture conditions, the cartilage did not lose the IGF-I responsiveness during prolonged culture periods.
ALP was also increased 2.5 times in hOBs grown on POL/nHA (p < 0.01), suggesting an increase in differentiation of these cells under the current culture conditions.
Inhibition of Lyn by continuous exposure to PCI-32765 as might occur under the current culture conditions [ 19] and may have added to the potency of the suppression of FcεRI degranulations.
These data suggest that n-telo-peptides of pro-collagen type II under the current culture condition are almost exclusively released during synthesis and thereby may be valid markers for collagen type II formation.
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