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Activity of the R292K rNA peaked at pH 5.1 and increased by 5-fold compared with that measured under conventional assay conditions (pH 6.5).
These findings indicate that the R292K virus population could be concealed because of its reduced enzymatic activity under conventional assay conditions.
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By optimizing assay conditions for three enzymes using Taguchi's method, 100 to 1000-fold higher sensitivity was achieved for cDNA synthesis than conventional assay condition using only RT.
We determined clonogenicity under conventional conditions and under conditions supplemented with growth factors and hormones that target breast tissue.
Age- and sex-matched groups of conventional NC/Nga male mice were separately purchased from Japan SLC and maintained separately under conventional conditions or SPF conditions.
In contrast to prior observations33,34, we found no inhibition of C. albicans growth under these assay conditions (Fig. 5c).
The residual enzyme activity was assayed under standard assay conditions.
The residual enzyme activity was then assayed under standard assay conditions.
The residual activity of modified enzyme was determined under standard assay conditions.
The aliquots were removed for the determination of residual enzyme activity under standard assay conditions.
The residual activity was subsequently measured under standard assay conditions (see above).
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