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Pre-formed spherical microtissues are loaded into the microchambers and cultured under continuous perfusion.
The bioreactors with an array of multiple wells favoring the maintenance of the three-dimensional (3-D) liver tissue cultures under continuous perfusion have been developed.
Her haemodynamic status remained stable under continuous perfusion of norepinephrine during and after SAE.
Isolated SH-SY5Y cells were selected and recorded under continuous perfusion with control medium (132 mM NaCl, 2 mM KCl, 1 mM CaCl2, 2 mM MgCl2, 1 mM Na-Gluconate, 10 mM HEPES and 10 mM d-glucose).
All whole cell patch clamp recordings were performed under continuous perfusion.
All recordings were obtained under continuous perfusion of oxygenated ACSF at 30°C.
Similar(46)
Measurement of fluorescence ratios under a continuous perfusion with normal saline solution was carried out for 5 min to confirm the stable basal level before applying any chemical.
Release profiles and real-time confocal imaging demonstrate that the fusions were retained within fibrin matrices, even under the stringent continuous perfusion conditions used in the latter.
Intracellular Ca2+-transients were analyzed in parallel with force under electrical stimulation and continuous perfusion using a novel setup as described previously [ 35].
Flow-induced shear stresses have been found to be a stimulatory factor in pre-osteoblastic cells seeded in 3D porous scaffolds and cultured under continuous flow perfusion.
A microfluidic continuous perfusion system was designed and tested to culture microalgae, Chlamydomonas reinhardtii, under constant nutrient concentration slightly lower than normal condition.
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