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Ramnolipids were extracted with diethyl ether under continuous agitation for 12 h.
The bottles were incubated in a BACTEC System at 37°C under continuous agitation up to 10 days.
The bottles were incubated in a BACTEC 9120 System (Becton Dickinson Microbiology Systems, Maryland, DE, USA) up to 10 days at 37°C under continuous agitation.
Stainings were performed on free-floating sections under continuous agitation.
The mixtures were incubated overnight at 4°C under continuous agitation.
AChE586-599 seeds were prepared by incubating 200 µM AChE586-599 in PBS for 3 hours under continuous agitation.
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Readings were taken automatically every 50 min for 92 h under conditions of continuous agitation at 37 °C, using four and three separate samples for the acetylated and nonacetylated aS, respectively.
Open image in new window Fig. 2 Six digesters under continuous orbital agitation (120 rpm).
The six reactors were incubated in a warm room at 35 ± 1 °C under continuous orbital agitation (120 rpm) (Chun et al. 2015; Vindis et al. 2009).
Incubation of the sections in primary and secondary antibodies was performed under continuous, gentle agitation at 4 °C.
This would suggest the ability to form aggregates under these conditions is occurring slower than samples incubated at their Tm with continuous agitation of 300 rpm.
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