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Deposition was done in e-beam evaporator at a 45∘ angle to taped surface under constant rotation of the insert about the deposition direction.
The sample thickness was also found to have a pronounced effect on the cloud point under constant rotation speed, where the immiscibility region in the cloud point shear rate diagram seemed broader with increasing sample thickness.
To the supernatant, 30 µl of Ni-NTA Agarose (Qiagen) was added and the mixture was incubated under constant rotation for 30 min at room temperature.
To 500 µl of the lysate, 5 µl of anti-Flag-M2 antibodies (Sigma) were added to the lysate and the mixture was incubated under constant rotation over night at 4°C.
Briefly, the lysate was cleared by centrifugation and then incubated with streptavidin sepharose for 2 hours at 4°C under constant rotation, washed three times with the provided streptavidin binding buffer and was then eluted with streptavidin elution buffer.
Immunoprecipitation was performed overnight at 4°C under constant rotation.
Similar(29)
After removing capsule and epididymis, the tubules were digested using DMEM/F-12 containing 1 mg/ml hyaluronidase (Sigma), 1 mg/ml collagenase (Sigma) and 15 U/ml DNase (Roche) for 35 40min at 37°C under constant mild rotation.
The rotating wall vessel (RWV) prevents cells from settling via a constant rotation.
"OK Computer" remains in constant rotation on our tour bus.
I keep the song "Good to Sea" on constant rotation.
Its constant rotation ensured that his face would always be lit by the sun.
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