The six replicates were kept under alternating light and dark conditions.
Fig. 5 a The schematic energy diagram of the MoS2 photodetector b the structure of MoS2 photodetector device Fig. 6 Current-voltage curves of the device a under a 150-W Xe lamp light source illumination and b in dark Fig. 7 a The output signal of photocurrent under alternating light on and light off, where the entire device was illuminated by a 150-W Xe lamp irradiation.
For the data from Stöckel et al. [ 16], the majority of genes under alternating light and dark cycles oscillate with a period of approximately 24 hours.
The genes in group 1 continued to oscillate under continuous light and were classified as circadian controlled genes, whereas genes from group 2 were light responsive and showed oscillating expression only under alternating light and dark cycles.
For the determination of cyclic behavior, a Fourier score cutoff of 6.5 corresponding to a False Discovery Rate of 2% under alternating light and dark conditions and of 3% under continuous light was used.
In contrast, microarray analyses of Cyanothece 51142 under alternating light and dark conditions revealed that ~30% of the genes in the genome are significantly diurnally regulated [ 16], whereas only 10% of these genes are circadian controlled [ 17].
The cultures were grown for 5 6 days under alternating light and dark conditions prior to collecting samples for every 4 hours over a time period of 2 days, starting at the transition from dark to light (time point L0) for 24 hours followed by 24 hours of continuous light.
After the application of both criteria, our analysis of the Stöckel et al. dataset [ 16] identified 43% of genes in the Cyanothece 51142 genome (2138 genes) with oscillating expression patterns under alternating light and dark conditions, in comparison to the previously reported 1445 genes.
Therefore, we combined and analyzed data from two independent microarray experiments, previously performed under alternating light-dark and continuous light conditions in Cyanothece sp. ATCC 51142, and sought to classify light responsive and circadian controlled genes.
This issue was addressed by the direct visualization of D96N bR under alternate illumination of green and blue light.
