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After 72 h incubation, the neurite outgrowth was assessed under a phase contrast microscope.
Cell morphologies were observed under a phase contrast microscope (Nikon Eclipse TS100, Tokyo, Japan) at predetermined time intervals.
Total cell numbers were counted under a phase contrast microscope at 400× magnification in a hemocytometer.
Colonies showing undifferentiated morphologies were then mechanically separated from the culture under a phase contrast microscope.
Five random fields at 40X fields were counted for each condition under a phase contrast microscope.
Cells in the flask were examined under a phase contrast microscope every 2 or 3 days.
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Continues aid beyond June 1 for those who qualify under a phase-out provision that was not part of the program that expired on Dec. 28.
Sections (4-µm) were stained with H&E and analyzed under a phase-contrast light microscope.
Appressoria penetration and infectious hyphal growth were monitored daily and photographed under a phase-contrast microscope.
Then the mixture was examined immediately under a phase-contrast microscope.
After incubation for 12, 24 and 36 hrs, the cells were photographed under a phase-contrast microscope.
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