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The plants were grown in a glasshouse with both natural light and additional artificial lighting when the natural light was below 25 kLux and kept under a cycle of 16 h light (22°C) and 8 h dark (20°C).
Animals were housed in a specific pathogen free facility at 22±2°C under a cycle of 12 hr light (7:00 am light on) and 12 hr dark (7:00 pm light off).
The animals were maintained at 23°C ± 2°C under a cycle of 12 h light/12 h darkness with free access to food and water.
Flasks were incubated in static cultures for 1 month at 24°C, under a cycle of 16 h light/8 h darkness.
They were kept under a cycle of 12 hours light : 12 hours dark (photophase from 7.00 a.m. to 7.00 p.m., with fluorescent lighting of 500 lux intensity at the cage floor level).
After successful transformation, 6 proven transgenic primary regenerants were grown to maturity in a greenhouse under a cycle of 16 h illumination and 8 h of darkness at 23 °C and 18 °C, respectively till grain maturity and for further analysis.
Similar(50)
The poor people of under developed nations experience a cycle where under nutrition and repeated infections lead to excess morbidity that can continue from generation to generation.
This has led to a cycle of under-performance.
They were slow to realise that after winning the treble under Jose Mourinho in 2010, a cycle came to an end.
James Jolly: It's funny that we should be talking about Beethoven symphonies because this evening the City of Birmingham Symphony Orchestra are starting a cycle under Andriss Nelsons.
Under a light dark cycle, R. palustris survived and maintained their H2 production activity in the continuous operating system.
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