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All the tests were performed in a temperature-controlled room at 28°C, under a continuous light regime.
Dried cysts were performed as indicated above, and incubated (1 g cyst per liter) in a hatcher at 28 30°C with strong aeration, under a continuous light regime.
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Because our experiment treated the whole seedling in a chamber under continuous light, there is a possibility that photosynthesis related genes were induced because roots were exposed to light even though they were covered by a layer of tissue and aluminum foil.
Plants were grown in a growth chamber under the continuous light at 28°C.
Interestingly, in the lwd1 lwdoubleble mutant, the expression of CCA1 and LHY also shows a reduced degree of oscillation under continuous light conditions and an arrhythmic expression pattern under constant dark conditions.
Plates were placed at 4°C for 3 d and then incubated in a growth chamber under continuous light at 23°C.
adjusted to a given temperature under continuous light at 120 150 μmol m-2 s-1.
Control plants were then grown in a growth chamber under continuous light (20 40 μm sec-1 m-2) at 23°C.
Interestingly, the mutant on site N8 exhibited growth rates slightly higher than the parental strain under continuous light, a feature that may be advantageous for biotechnological applications.
Plates were incubated at 4°C for 2 days in the dark, and then placed in a climate chamber under continuous light (60 µmol photons m−2 s−1) at 22°C.
Strains PCC7811 and PCC7821 were grown at 20°C in a BG11 medium under continuous light (5 to 15 μmol photons m-2 s-1) and the cells were harvested during logarithmic growth.
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